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Figure 2 | BMC Biotechnology

Figure 2

From: CpG-island fragments from the HNRPA2B1/CBX3genomic locus reduce silencing and enhance transgene expression from the hCMV promoter/enhancer in mammalian cells

Figure 2

FACScan analysis of EGFP expressing CHO-K1 cells. Following stable transfection with hCMV-EGFP, 8.0 kb RNP-EGFP, or 4.0 kb RNP-EGFP, pools of >100 transfectants were cultured continuously with drug selection for 200 generations, and FACScan analysis conducted after 33 and 200 generations. The results are plotted as green fluorescence on a logarithmic scale (FL1-H) versus number of events on a linear scale (counts). Figures for gate M1 are % cells with undetectable fluorescence; those for gate M2 are % cells showing greater than 1000 fluorescence units.

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BMC Biotechnology

ISSN: 1472-6750

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