Table 1 QTL Lightspeed™ Protein Assay Statistical Data18. Statistics are shown for Z' Factor, signal/noise (S/N), signal/background (S/B), signal window (SW) and coefficient of variation (% CV) at maximal substrate phosphorylation. Z'Factor equal to or greater than 0.5 are an indication of a robust assay18. Ten replicate measurements were made to determine the assay statistics.
From: High-throughput kinase assays with protein substrates using fluorescent polymer superquenching
Protein | Enzyme | Z' Factor | S/N | S/B | SW | %CV |
|---|---|---|---|---|---|---|
MBP | PKCα | 0.84 | 20.7 | 2.6 | 33.4 | 2.2 |
MBP | IRAK4 | 0.80 | 23 | 2.6 | 31 | 4.3 |
PHAS-1 | PKCα | 0.52 | 9.6 | 0.7 | 9.6 | 2.8 |
Histone H1 | PKCα | 0.43 | 7.32 | 0.7 | 9.4 | 3.0 |