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Table 1 QTL Lightspeed™ Protein Assay Statistical Data18. Statistics are shown for Z' Factor, signal/noise (S/N), signal/background (S/B), signal window (SW) and coefficient of variation (% CV) at maximal substrate phosphorylation. Z'Factor equal to or greater than 0.5 are an indication of a robust assay18. Ten replicate measurements were made to determine the assay statistics.

From: High-throughput kinase assays with protein substrates using fluorescent polymer superquenching

Protein Enzyme Z' Factor S/N S/B SW %CV
MBP PKCα 0.84 20.7 2.6 33.4 2.2
MBP IRAK4 0.80 23 2.6 31 4.3
PHAS-1 PKCα 0.52 9.6 0.7 9.6 2.8
Histone H1 PKCα 0.43 7.32 0.7 9.4 3.0