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Table 1 Comparison of repeatability estimates of the PCR measurements using different reaction mixes and platforms over three days (using standard deviations of the Ct value).

From: An inter-platform repeatability study investigating real-time amplification of plasmid DNA

Repeatability Estimates (standard deviations of the Ct value) based on Individual Plasmid Copy Number

Plasmid Copy Number

Platform and PCR Reaction Mix

 

ABI 7700

LightCycler

RotorGene

 

Excite

FastStart

Excite

FastStart

Excite

FastStart

102

0.543

0.182

0.568

0.233

0.888

0.186

103

0.452

0.204

1.177

0.190

0.184

0.445

104

0.223

0.305

1.123

0.100

0.256

0.592

105

0.243

0.168

0.947

0.224

0.209

0.221

106

0.362

0.183

1.378

0.281

0.772

0.333

107

0.473

0.189

0.919

0.156

0.319

0.247

108

0.236

0.184

0.454

0.352

0.322

0.166

Degrees of Freedom

6–8

6

6–8

5–6

6–8

6

Mean

0.362

0.202

0.938

0.219

0.421

0.313

Standard Deviation

0.130

0.047

0.331

0.082

0.286

0.156

  1. (Note that one replicate is missing for the LC platform coupled with the Fast Start reaction mix – the capillary broke).