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Figure 4 | BMC Biotechnology

Figure 4

From: Simultaneous imaging of GFP, CFP and collagen in tumors in vivousing multiphoton microscopy

Figure 4

Differences in motility and invasion can be determined by imaging cells with different metastatic potential in the same tumor. A) The panels show a time series of images of an experimentally generated mammary tumour with cells either expressing GFP (low metastatic in green) or CFP (high metastatic in white) with collagen fibres in purple. Arrows point to moving CFP cells and arrowheads to moving GFP cells. Supplementary movie shows this image sequence including intermediate frames (Additional file 1). Images were taken with a 20× objective and a final magnification of 250×. B) CFP-labeled cells (white) and control GFP labelled cells (green) are seen in the lung after extravasation and metastatic growth. Images were taken with a 20× objective and a final magnification of 250×. C) Left hand panel CFP cell (white) has a filopod (arrow) protruding into a field of GFP control cells (green). Right hand panel CFP cell (white) has a lamellapod (arrow) seen in the middle of a field of GFP control cells (green). Supplementary movie shows a lamellapod retracting over time (Additional file 2). Images were taken with a 60× objective and a final magnification of 1000×. D) The panels show a time series of high magnification images of an experimentally generated mammary tumor with cells either expressing GFP (in green) or CFP (in white) with collagen fibers in purple. Moving high metastatic cells are outlined in white and an orange arrow shows the path taken by both cells. Supplementary movie shows this image sequence including intermediate frames (Additional file 3). Images were taken with a 40× objective and a final magnification of 500×. Scale bar for A-E = 25 um.

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