Figure 2

Specificity of oligodeoxyribonucleotide hybridization to PNA probes. Hybridizations were performed at room temperature with a concentration of 7.5 × 10¹⁰ targets per μL using the fluorescent cationic polymer for detection. Hybridization of PNA probes to perfectly complementary, or complementary oligonucleotides presenting a terminal mismatch, a central mismatch, or two mismatches were performed in triplicate. Fluorescence intensities from hybridized probes were corrected by substraction of background fluorescence intensity.