Figure 1
From: A gene expression system offering multiple levels of regulation: the Dual Drug Control (DDC) system
A. Schematic of system. DBD (ZFHD) and AD (p65) fused components are co-expressed either as a single unit from one CMV promoter using an IRES element, or are separated to two different plasmids (DBD from pAr-DBD and AD from pAr-AD). In the presence of dimerizer, these elements activate expression of a ZFHD-responsive reporter (hGH or SEAP) and the tTA protein. In the absence of tetracycline, tTA activates expression of gene 2 (SEAP or GFP).