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Table 2 Kinetic and binding parameters for three scFvs selected from the Nkuku library1.

From: A large semi-synthetic single-chain Fv phage display library based on chicken immunoglobulin genes

scFv

k a (1/Ms)

k d (1/s)

K D (M)

anti-F-BSA

7 × 106

1.68 × 10-2

2.4 × 10-9

anti-CYT

4.29 × 106

3.64 × 10-3

3.5 × 10-10

anti-KLH

2.41 × 106

3.64 × 10-3

2.83 × 10-9

  1. 1Association and dissociation rate constants were measured using BIACORE. Each value represents the average of two independent determinations on the same chip surface.
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BMC Biotechnology

ISSN: 1472-6750

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