Figure 4
From: A large semi-synthetic single-chain Fv phage display library based on chicken immunoglobulin genes
Sandwich ELISA for virus detection. Double antibody sandwich ELISA absorbance values obtained in which dilutions of (A) AHSV or (B) BTV particles were trapped from suspension using virus-specific rabbit IgG and detected using phage-displayed scFvs (clone Lca12 for AHSV, clone H2 for BTV; see Figure 3) as the second antibody in the sandwich. Binding phages were detected with a pVIII-specific mAb and a peroxidase-labelled anti-mouse IgG immunoglobulin. Each point represents the average of three determinations. Error bars show one standard deviation from the mean.