Figure 1

Amino acid sequences of VH CDR3s. Amino acid sequences of scFv clones showing the VH CDR3s and their flanking regions in the naïve (A) and the semi-synthetic (B) repertoires which were combined to make up the final Nkuku recombinant antibody library. The clones from the naïve library were randomly picked while representatives from each predetermined size class are shown in the case of the synthetic repertoire. Identical amino acid residues are shown by dashes (-) while dots (.) indicate spaces introduced to align sequences.