Table 4 Expression levels of mammalian proteins expressed with N-terminal H10-MBP fusions, with selected protein features
No. | Protein | Accession No. | PfamA Domaina | Construct | MW (kDa) | hp_aa | LC | Total Expression (mg / l) | Soluble Expression (mg / l) |
|---|---|---|---|---|---|---|---|---|---|
31 | TAL1 | P17542 | HLH | 179–331/331 | 16.5 | 3.0 | 1.0 | 13.2 | 8.3 |
32 | ELF1 | P32519 | Ets | 2–619/619 | 67.4 | 6.0 | 5.0 | 0.0 | 0.0 |
33 | ELF1 | P32519 | nab | 2–167/619 | 18.0 | 4.0 | 2.0 | 14.6 | 14.6 |
34 | ELF1 | P32519 | Ets | 204–619/619 | 45.4 | 6.0 | 2.0 | 0.0 | 0.0 |
35 | ELF1 | P32519 | na | 316–619/619 | 32.2 | 3.0 | 1.0 | 0.0 | 0.0 |
36 | ELF1 | P32519 | Ets | 204–306/619 | 12.1 | 6.0 | 0.0 | 65.0 | 19.3 |
37 | Elf1 | Q60775 | Ets | 2–612/612 | 66.1 | 6.0 | 4.0 | 24.0 | 23.5 |
38 | Elf1 | Q60775 | Ets | 2–306/612 | 34.0 | 6.0 | 3.0 | 60.6 | 27.2 |
39 | Elf1 | Q60775 | na | 2–167/612 | 17.8 | 4.0 | 2.0 | 8.6 | 8.4 |
40 | Elf1 | Q60775 | Ets | 204–612/612 | 44.2 | 6.0 | 1.0 | 15.5 | 10.4 |
41 | Elf1 | Q60775 | Ets | 204–306/612 | 12.1 | 6.0 | 0.0 | 18.5 | 12.1 |
42 | Elf1 | Q60775 | na | 316–612/612 | 30.7 | 3.0 | 0.0 | 0.0 | 0.0 |
43 | Gata1 | P17679 | GATA × 2 | 2–413/413 | 42.5 | 3.0 | 6.0 | 16.0 | 14.3 |
44 | Gata1 | P17679 | GATA × 2 | 2–319/413 | 33.8 | 3.0 | 5.0 | 67.0 | 15.8 |
45 | Gata1 | P17679 | na | 2–182/413 | 18.6 | 3.0 | 3.0 | 9.7 | 7.2 |
46 | Gata1 | P17679 | GATA × 2 | 191–413/413 | 23.1 | 3.0 | 5.0 | 19.7 | 9.6 |
47 | Gata1 | P17679 | GATA × 2 | 191–319/413 | 14.3 | 3.0 | 0.0 | 0.0 | 0.0 |
48 | Gata2 | O09100 | GATA × 2 | 2–480/480 | 50.3 | 5.0 | 5.0 | 0.0 | 0.0 |
49 | Gata2 | O09100 | na | 2–189/480 | 19.4 | 4.0 | 2.0 | 0.0 | 0.0 |
50 | Gata2 | O09100 | GATA × 2 | 275–480/480 | 22.4 | 5.0 | 2.0 | 0.0 | 0.0 |
51 | Gata2 | O09100 | GATA × 2 | 275–402/480 | 14.2 | 1.0 | 1.0 | 0.0 | 0.0 |
52 | Fli1 | P26323 | SAM_PNT, Ets | 2–452/452 | 50.9 | 3.0 | 1.0 | 0.0 | 0.0 |
53 | Fli1 | P26323 | SAM_PNT, Ets | 2–363/452 | 41.7 | 3.0 | 0.0 | 134.5 | 61.0 |
54 | Fli1 | P26323 | SAM_PNT | 2–198/452 | 22.2 | 3.0 | 0.0 | 121.2 | 86.8 |
55 | Fli1 | P26323 | SAM_PNT, Ets | 114–452/452 | 38.6 | 3.0 | 1.0 | 61.0 | 38.2 |
56 | Fli1 | P26323 | SAM+ETS | 114–363/452 | 29.4 | 3.0 | 0.0 | 96.5 | 73.1 |
57 | Fli1 | P26323 | SAM_PNT | 114–196/452 | 10.0 | 3.0 | 0.0 | 71.8 | 51.7 |
58 | Fli1 | P26323 | Ets | 280–452/452 | 20.1 | 3.0 | 1.0 | 28.6 | 16.3 |
59 | Fli1 | P26323 | Ets | 280–363/452 | 10.9 | 3.0 | 0.0 | 23.4 | 23.0 |
60 | Lmo2 | P25801 | LIM × 2 | 2–158/158 | 18.2 | 3.0 | 0.0 | 106.7 | 23.8 |
61 | Ldb1 | P70662 | LIM_bind | 2–375/375 | 42.6 | 3.0 | 2.0 | 0.0 | 0.0 |
62 | Ldb1 | P70662 | LIM_bind | 2–273/375 | 31.9 | 3.0 | 0.0 | 133.8 | 62.0 |
63 | Ldb1 | P70662 | LIM_bind | 275–375/375 | 10.5 | 2.0 | 1.0 | 2.0 | 1.7 |
64 | Lyl1 | P27792 | HLH | 40–278/278 | 26.2 | 3.0 | 0.0 | 3.8 | 2.6 |
65 | Lyl1 | P27792 | HLH | 40–215/278 | 19.6 | 3.0 | 0.0 | 4.2 | 2.5 |
66 | Lyl1 | P27792 | na | 40–135/278 | 10.3 | 3.0 | 0.0 | 3.6 | 1.7 |
67 | Lyl1 | P27792 | HLH | 150–278/278 | 14.8 | 3.0 | 0.0 | 40.1 | 32.2 |
68 | Lyl1 | P27792 | HLH | 150–215/278 | 8.2 | 3.0 | 0.0 | 60.3 | 20.8 |
69 | Ttr | P07309 | transthyretin | 20–147/147 | 13.6 | 5.0 | 0.0 | 59.2 | 49.7 |
70 | Pin1 | Q9QUR7 | WW, Rotamase | 2–163/163 | 18.2 | 2.0 | 0.0 | 36.9 | 19.4 |
71 | Whsc1 | Q7TSF5 | PHD × 2, PWWP, SET | 2–558/558 | 63.8 | 4.0 | 2.0 | 9.4 | 1.3 |
72 | Whsc1 | Q7TSF5 | PHD, PWWP, SET | 2–373/558 | 43.0 | 4.0 | 0.0 | 21.6 | 12.4 |
73 | Whsc1 | Q7TSF5 | PHD, PWWP | 2–149/558 | 17.2 | 4.0 | 0.0 | 0.0 | 0.0 |
74 | Whsc1 | Q7TSF5 | PWWP, SET, PHD | 70–558/558 | 56.1 | 4.0 | 2.0 | 5.1 | 2.1 |
75 | Whsc1 | Q7TSF5 | PWWP, SET | 70–373/558 | 35.3 | 4.0 | 0.0 | 18.2 | 17.9 |
76 | Whsc1 | Q7TSF5 | PWWP | 70–149/558 | 9.5 | 4.0 | 0.0 | 56.8 | 14.6 |
77 | Whsc1 | Q7TSF5 | SET | 249–373/558 | 14.3 | 4.0 | 0.0 | 34.7 | 22.6 |
78 | Maat1 | NM_024227 | na | 2–257/257 | 30.0 | 3.0 | 0.0 | 2.7 | 2.4 |
79 | BC031407 | NM_145596 | na | 2–630/630 | 67.3 | 6.0 | 6.0 | 0.0 | 0.0 |
80 | BC031407 | NM_145596 | na | 2–455/630 | 48.5 | 6.0 | 5.0 | 0.0 | 0.0 |
81 | BC031407 | NM_145596 | na | 2–179/630 | 19.4 | 4.0 | 1.0 | 9.6 | 8.7 |
82 | BC031407 | NM_145596 | na | 178–630/630 | 48.0 | 6.0 | 5.0 | 0.0 | 0.0 |
83 | BC031407 | NM_145596 | na | 178–455/630 | 29.1 | 6.0 | 4.0 | 0.0 | 0.0 |
84 | BC031407 | NM_145596 | na | 413–630/630 | 23.7 | 4.0 | 1.0 | 0.0 | 0.0 |
85 | Bzrp2 | P50637 | TspO_MBR | 2–169/169 | 18.7 | 4.0 | 0.0 | 0.0 | 0.0 |
86 | MGC19339 | NM_145954 | Aldedh | 40–486/803 | 47.0 | 5.0 | 2.0 | 37.2 | 18.7 |
87 | Bsg | NM_009768 | Ig × 2, V-set | 28–323/389 | 32.4 | 4.0 | 0.0 | 61.2 | 36.6 |
88 | Snx15 | NM_026912 | PX, MIT | 2–337/337 | 37.6 | 4.0 | 1.0 | 32.4 | 32.0 |
89 | Snx15 | NM_026912 | PX | 2–226/337 | 25.6 | 4.0 | 1.0 | 20.0 | 18.8 |
90 | Atp2b2 | Q9R0K7 | Cation_ATPase_N | 2–94/1198 | 10.4 | 2.0 | 0.0 | 42.1 | 24.7 |
91 | Atp2b2 | Q9R0K7 | Cation_ATPase_N | 1039–1198/1198 | 17.9 | 2.0 | 3.0 | 6.1 | 5.0 |
92 | cdh23 | Q99PF4 | Cadherin | 33–132/3354 | 11.1 | 4.0 | 0.0 | 174.7 | 38.8 |
93 | Myo15 | Q9QZZ4 | SH3_2 | 2847–2937/3511 | 9.8 | 4.0 | 0.0 | 8.6 | 0.0 |
94 | Myo7a | P97479 | SH3_1 | 1602–1672/2215 | 7.8 | 4.0 | 0.0 | 76.0 | 35.4 |
95 | tmc1 | Q8R4P5 | na | 2–193/757 | 22.9 | 3.0 | 3.0 | 0.0 | 0.0 |
96 | Trvp4 | NM_022017 | na | 500–718/871 | 24.6 | 9.0 | 0.0 | 0.0 | 0.0 |
97 | Whrn | XM_196324 | PDZ | 811–908/908 | 11.0 | 3.0 | 0.0 | 34.1 | 31.1 |
98 | Espn | NM_019585 | WH2 | 2–253/253 | 28.0 | 3.0 | 2.0 | 5.5 | 3.8 |
99 | Map2 | P20357 | Tubulin-binding | 1657–1755/1828 | 10.6 | 2.0 | 0.0 | 47.1 | 46.8 |
100 | Prom | O54990 | Prominin | 124–162/867 | 4.3 | 2.0 | 1.0 | 16.3 | 8.5 |
101 | GluR1 | P23818 | ANF_receptor | 19–538/907 | 59.0 | 4.0 | 0.0 | 0.0 | 0.0 |
102 | GluR2 | P23819 | ANF_receptor | 22–545/883 | 58.6 | 4.0 | 0.0 | 0.0 | 0.0 |
103 | Grin1 | P35438 | na | 834–938/938 | 12.0 | 5.0 | 0.0 | 13.8 | 13.8 |
104 | Grin2a | P35436 | na | 23–555/1464 | 59.9 | 6.0 | 0.0 | 58.5 | 8.2 |
105 | Grin2b | Q01097 | Lig_chan | 656–817/1482 | 18.1 | 4.0 | 0.0 | 0.0 | 0.0 |
106 | Dlgh2 | NM_011807 | PDZ | 419–530/852 | 11.7 | 4.0 | 0.0 | 13.8 | 13.7 |
107 | Dlgh4 | Q62108 | PDZ | 311–394/724 | 8.7 | 4.0 | 0.0 | 37.4 | 29.2 |
108 | Dlgh3 | P70175 | PDZ | 402–509/849 | 11.7 | 5.0 | 0.0 | 0.0 | 0.0 |
109 | Dlgh1 | U93309 | PDZ | 432–572/927 | 15.1 | 5.0 | 0.0 | 26.7 | 23.4 |
110 | Syngap1 | XM_139847 | RasGAP | 405–615/1318 | 23.9 | 3.0 | 0.0 | 0.0 | 0.0 |
111 | Grip1 | Q925T5 | PDZ | 1–112/1034 | 9.6 | 3.0 | 0.0 | 0.0 | 0.0 |
112 | Homer1 | Q9Z2Y3 | WH1 | 2–107/354 | 12.1 | 3.0 | 0.0 | 17.9 | 17.6 |
113 | Homer3 | Q99JP6 | WH1 | 2–110/356 | 39.3 | 4.0 | 0.0 | 0.0 | 0.0 |
114 | TtyhI | Q9EQN7 | na | 263–450/450 | 20.6 | 5.0 | 0.0 | 35.6 | 28.3 |
115 | 1500001H12RIKEXT2 | NM_021316 | na | 2–149/149 | 14.8 | 5.0 | 3.0 | 66.1 | 66.1 |
116 | Ext2 | NM_010163 | na | 99–392/718 | 33.0 | 4.0 | 0.0 | 18.5 | 3.5 |
117 | KIAA1136 | Q9ULT3 | na | 45–214/597 | 19.2 | 2.0 | 0.0 | 26.8 | 10.4 |
118 | G2 | Q12914 | na | 1046–1692/1692 | 71.3 | 5.0 | 3.0 | 0.0 | 0.0 |
119 | KIAA1549 | Q9HCM3 | na | 184–464/1865 | 29.5 | 5.0 | 4.0 | 3.5 | 0.0 |
120 | Nfkb1 | P25799 | RHD | 39–365/971 | 36.7 | 5.0 | 0.0 | 27.1 | 22.7 |
121 | Nfkb1 | P25799 | RHD, TIG, Ank × 6, Death | 2–971/971 | 105.5 | 7.0 | 3.0 | 0.0 | 0.0 |
122 | RelA-p65 | Q04207 | RHD, TIG | 18–306/549 | 32.9 | 4.0 | 0.0 | 24.1 | 18.2 |
123 | RelA-p65 | Q04207 | RHD, TIG | 2–549/549 | 60.0 | 5.0 | 2.0 | 0.0 | 0.0 |
124 | RelB | Q04863 | RHD, TIG | 102–418/558 | 35.8 | 4.0 | 0.0 | 46.0 | 25.9 |
125 | myog | P12979 | HLH, Basic | 2–224/224 | 25.1 | 3.0 | 1.0 | 25.8 | 12.4 |