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Table 1 Proteins for expression study with selected features

From: Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression

No

Proteina

Domainb

Constructc

Organismd

Protein Familye

MW (Kda)

pI

Cys %

GRAVYf

hp_aag

Sub-cellular Location

LCh

CCi

26

CASP2

FL

1–435/435

Hs

CARD, Peptidase_C14

48.9

6.3

4.1

-0.30

5

Cytoplasm

1

0

24

CCND2

FL

1–289/289

Hs

cyclin, cyclin_C

33.1

4.9

4.1

-0.21

4

Cytoplasm

2

0

29

CD44

FL

1–742/742

Hs

Xlink, Pfam-B × 9

81.6

5.0

1.2

-0.77

10

Extra-cellular

9

0

22

CDK2

FL

1–298/298

Hs

pkinase

33.9

8.9

1

-0.08

4

Cytoplasm

0

0

23

CDK4

FL

1–303/303

Hs

pkinase

33.7

6.6

1.3

-0.17

4

Cytoplasm

0

0

25

CDKN1B

FL

1–198/198

Hs

CDI, Pfam-B × 2

22.1

6.6

2

-1.26

2

Cytoplasm

0

1

28

CDKN2A

FL

1–156/156

Hs

ank

16.5

5.4

0.6

-0.23

4

Cytoplasm

0

0

6

Efna1

FL

18–205/205

Mm

Ephrin

21.9

6.4

2.1

-0.59

8

Extra-cellular

1

0

7

Efna1

EC

18–154/205

Mm

Ephrin

16.2

6.5

2.9

-0.86

2

Extra-cellular

0

0

5

Efnb2

EC1

29–176/336

Mm

Ephrin

16.6

5.3

2.7

-0.47

3

Extra-cellular

0

0

4

Efnb2

EC2

29–210/336

Mm

Ephrin

20.1

8.6

2.2

-0.64

3

Extra-cellular

0

0

15

EGFR

TK

694–1022/1210

Hs

Pkinase, Pfam-B

37.3

5.5

1.8

-0.22

3

Cytoplasm

1

0

8

Epha2

LB

24–206/977

Mm

EPH_lbd

21.1

4.7

2.7

-0.30

4

Extra-cellular

0

0

1

Ephb2

LB

28–210/994

Mm

EPH_lbd

22.5

5.8

2.2

-0.14

4

Extra-cellular

0

0

3

Ephb2

SAM

922–994/994

Mm

SAM_1

8.3

4.9

0

-0.03

2

Cytoplasm

0

0

2

Ephb2

TK

595–906/994

Mm

Pkinase

35.3

5.6

1.6

-0.27

5

Cytoplasm

0

0

10

Fli1

FL

1–452/452

Mm

Ets, SAM_PNT, Pfam-B × 5

51.0

6.6

0.9

-0.79

3

Nuclear

1

0

19

FOS

FL

1–380/380

Hs

bZIP, Pfam-B × 4

40.7

4.6

2.1

-0.37

5

Nuclear

5

1

9

GATA2

FL

1–480/480

Hs

GATA

50.3

9.7

2.7

-0.51

13

Nuclear

7

0

30

GFP

FL

1–238/238

Av

GFP

26.9

5.6

0.8

-0.52

3

Cytoplasm

0

0

14

GRB2

FL

1–217/217

Hs

SH2, SH3

25.2

5.9

0.9

-0.67

5

Cytoplasm

0

0

17

HRAS

FL

1–189/189

Hs

ras

21.3

5.0

3.2

-0.42

4

Cytoplasm

1

0

18

JUN

FL

1–331/331

Hs

bZIP, Jun

35.7

9.0

0.9

-0.47

3

Nuclear

3

1

20

MAD

FL

1–221/221

Hs

HLH, Pfam-B × 2

25.3

8.9

1.4

-0.97

2

Nuclear

3

1

21

MAX

FL

1–160/160

Hs

HLH, Pfam-B × 2

18.3

5.9

0

-1.32

2

Nuclear

1

1

12

Mdm2

FL

1–489/489

Mm

SWIB, zf-RanBP, Pfam-B × 8

54.5

4.5

3.5

-0.83

4

Nuclear / Cytoplasm

5

0

13

Mdm2

p53-bd

19–230/489

Mm

SWIB, Pfam-B × 2

11.7

8.8

0.5

-0.25

4

Nuclear / Cytoplasm

3

0

27

MMP1

FL

1–469/469

Hs

Peptidase_M10_N, Peptidase_M10, Hemopexin

54.0

6.5

0.6

-0.57

7

Extra-cellular

0

0

16

RAF1

Ras-bd

51–131/648

Hs

RBD

9.2

9.9

3.8

-0.30

3

Cytoplasm

0

0

11

Trp53

FL

1–390/390

Mm

P53

43.5

7.0

3.1

-0.59

3

Nuclear / Cytoplasm

1

0

  1. aLocusLink symbol. bDomain: LB, ligand binding; TK, tyrosine kinase; SAM, sterile alpha motif; EC, extra-cellular; FL, full-length; bd, binding domain. cConstruct expressed numbered by amino acid position (start – finish / total). dOrganism: Mm, Mus musculus; Hs, Homo sapiens; Av, Aequoria Victoria. eProtein family nomenclature according to the Pfam database http://www.sanger.ac.uk/Software/Pfam/. fGRAVY, grand average of hydropathicity index. gHighest number of contiguous hydrophobic amino acids (A, V, I, L, W or F). hLC and iCC, number of low complexity and coiled coil regions according to Pfam database.