Figure 3From: Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expressionEffect of C-terminal fusion on protein expression Total (T) and soluble (S) expression for protein 1 – 30 (Table 1) with different C-terminal fusion partners analysed by SDS-PAGE fluorescence western blots as Figure 2. Expression plasmids employed were (g) pET-DEST42, (h) pDEST-C101 or pDEST-C102 with either (i) MBP, (j) GST, (k) GFP (l) Trx (m) Dhfr or (n) DHFR inserted between the DraIII and BfrBI sites as shown in Figure 1.Back to article page