Figure 2

Effect of N-terminal fusion on protein expression Total (A) and soluble (B) expression for protein 1 – 30 (Table 1) with various N-terminal fusion partners analysed by SDS-PAGE fluorescence western blots as described in Materials and Methods. Expression plasmids employed were (a) pDEST17, (b) pDEST-N110 or pDEST-N112 with either (c) MBP, (d) GFP, (e) GST or (f) Trx inserted between the DraIII and BfrBI sites as shown in Figure 1.