Figure 2

The limitation on the dynamic range of log-ratio measurements, (A) shown here in the diamond-shaped gray-shaded box between the colored lines on an R-I plot, reflects the limited range of values that can be obtained from existing microarray technology which typically employ 16-bit array scanners that allow each channel on the arrays to produce measurements ranging in log₂ values from 0 through 16. (B) The diamond area represents a rotation of the original axes, x = log₂(G) and y = log₂(R) to new axes and , followed by a simple rescaling to x" = [log₂(R) + log₂(G)]/2 and y" = [log₂(R) - log₂(G)].