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Table 3 Comparison of the vectors LBmSN and LBSN upon BALB/MK and NIH3T3 cells

From: Over expression of the selectable marker blasticidin S deaminase gene is toxic to human keratinocytes and murine BALB/MK cells

 

BALB/MK

NIH3T3

 

BS1

No antibiotic

BS

No antibiotic

LBmSN

L

D

L

L

LBSN

L

L

L

L

No vector

D

L

D

L

  1. The experiment was carried out as described in the legend of the Figure 1. After 8 days of the transduction the cells were stained with Coomassie Blue for microscopic analysis. The results are representative of three independent experiments, which gave essentially the same results.
  2. 1 [BS] = In the media of the cells transduced with LBmSN and LBSN were added 8 μg/ml and 2 μg/ml of BS respectively, because the cells transduced with LBSN do not support concentrations of BS higher than 2 μg/ml [8].
  3. D = dead
  4. L = alive