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Table 3 Comparison of the vectors LBmSN and LBSN upon BALB/MK and NIH3T3 cells

From: Over expression of the selectable marker blasticidin S deaminase gene is toxic to human keratinocytes and murine BALB/MK cells

  BALB/MK NIH3T3
  BS1 No antibiotic BS No antibiotic
LBmSN L D L L
LBSN L L L L
No vector D L D L
  1. The experiment was carried out as described in the legend of the Figure 1. After 8 days of the transduction the cells were stained with Coomassie Blue for microscopic analysis. The results are representative of three independent experiments, which gave essentially the same results.
  2. 1 [BS] = In the media of the cells transduced with LBmSN and LBSN were added 8 μg/ml and 2 μg/ml of BS respectively, because the cells transduced with LBSN do not support concentrations of BS higher than 2 μg/ml [8].
  3. D = dead
  4. L = alive
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BMC Biotechnology

ISSN: 1472-6750

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