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Figure 7 | BMC Biotechnology

Figure 7

From: Over expression of the selectable marker blasticidin S deaminase gene is toxic to human keratinocytes and murine BALB/MK cells

Figure 7

Effect of BS upon the keratinocytes transduced with LBmSN and selected with BS The keratinocytes were cultured and selected with 8 μg/ml BS as described in Figure 5. When the keratinocytes reached 70 % confluence, the cells were divided into two parts and transferred to 2 wells of a 12-well plate that contained the irradiated NIH3T3 cells. In one well the cells were maintained without BS (A) and in another with 8 μg/ml BS (B). The cells were stained with Rhodamine B after 10 days.

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