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Figure 5 | BMC Biotechnology

Figure 5

From: Mucosal delivery of anti-inflammatory IL-1Ra by sporulating recombinant bacteria

Figure 5

IL-1Ra-producing B. subtilis protects mice from endotoxic shock. LPS-sensitive C3H/HeOuJ mice (29–52 mice/group tested in four separate experiments) received 3 × 106 pSM539 bacteria (engineered with human mature IL-1Ra; ) i.p. 24 h before administration of LPS (15–20 mg/kg). Mice were observed for 96 h and deaths recorded. Control mice received either the B. subtilis strain pSM214 (β-lactamase control;) or PBS (×) 24 h before LPS administration. Statistical analysis of survival curves (χ2) indicated a significant increase in survival of mice receiving pSM539 as compared to controls receiving PBS or receiving pSM214. At 16 h difference was statistically significant at p < 0.05 vs. pSM214 but not significant vs. PBS (+). From 24 to 40 h, difference was statistically significant only vs. pSM214 at p < 0.01 (#). From 44 h on, difference was statistically significant vs. both controls at p < 0.01 (*). Comparison of survival percentiles showed a statistically significant difference (p < 0.01) between pSM539 and control groups pSM214 and PBS at 75% and 50% percentiles. Survival of control mice receiving pSM214 was never statistically different from that of mice receiving PBS (p > 0.2).

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