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Table 1 Microinjection of reporter vector.

From: Efficient activation of gene expression using a heat-shock inducible Gal4/Vp16-UAS system in medaka

    

Reporter expression

Vector

MN

Host

Fish

before induction1

after induction2 (mosaic)

after induction2 (uniform)

pCG5.0WCS pCG3.0Y

+

wildtype

74

52

61/61 (82%)

12/12 (16%)

pCG3.0Y

-

pCG6.0WCS/T3

162

29

-/-

73/73 (100%)

  1. 1...Leaky expression was mainly observed in the yolk only
  2. 2...Expression of internal (CFP) and independent (YFP) effector in the embryonic body was scored separately; CFP/YFP
  3. 3...Injection was performed in offspring of heterozygous carriers mated to wildtype fish, resulting in ~50% transgenic embryos
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BMC Biotechnology

ISSN: 1472-6750

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