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Figure 8 | BMC Biotechnology

Figure 8

From: Enhanced cell-permeant Cre protein for site-specific recombination in cultured cells

Figure 8

Cre-mediated recombination in primary splenocytes. Splenocytes from ROSA-26R mice were treated with 0 (thin black line), 1.0 (light grey line), 2.0 (dark grey line) and 5.0 (thick black line) μM HNC recombinase in serum free media for 2 hours. The cells were washed, cultured for 24 hours, stained with a fluorescent β-galactosidase substrate (ImaGene green™, C12FDG) and analyzed by flow cytometry. Cre-mediated recombination, which activates the expression of a floxed lacZ gene, results in increased fluorescence.

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