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Figure 7 | BMC Biotechnology

Figure 7

From: Enhanced cell-permeant Cre protein for site-specific recombination in cultured cells

Figure 7

Cre-mediated recombination in different cell types. Cos7 and NIH3T3 cells containing a single integrated copy of the pBABE.lox.stp.EGFP retrovirus and murine embryonic stem (ES) cells containing a floxed IKK γ gene were treated with 0, 0.5, 1.0, 2.0, 5.0, and 10 μM HNC (Cos7 and NIH3T3) or with 0, 0.25, 0.5, 1.0, 2.0, and 5.0 μM HNC (ES cells) for two hours. Cellular DNA was extracted, digested with EcoRI, and analyzed by Southern blot analysis. Cre-mediated recombination results in the conversion of the loxP-containing fragment (upper band, U) to the recombination product (lower band, R).

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