Skip to main content
Figure 5 | BMC Biotechnology

Figure 5

From: Enhanced cell-permeant Cre protein for site-specific recombination in cultured cells

Figure 5

Uptake of fluorescent Cre fusion proteins. Tex.loxp.EG cells were treated for different lengths of time with the indicated Alexa 488-labeled Cre fusion proteins at 4 or 37°C and protein uptake was monitored by flow cytometry. (A) Kinetics of protein uptake. The mean fluorescence of cells treated with Alexa 488-labeled Cre fusion proteins, as calculated by the CellQuest software, increased with time and was inhibited at 4°C. (B) Representative FACS profiles of cells treated with the fluorescent HC protein. Cells were treated with Alexa 488-labeled HNCM at the indicated temperature for 0 (light gray, solid), 15 (medium gray) and 120 min (black) and analyzed by flow cytometry. Profiles of cells treated for 30 and 60 min. have been omitted for clarity.

Back to article page