Figure 1
From: Enhanced cell-permeant Cre protein for site-specific recombination in cultured cells
Recombinant Cre fusion proteins. (A) Structures of recombinant Cre fusion proteins. Cre sequences from nucleotide 484 to 1513 (GenBank X03453) were expressed unaltered or incorporated into fusion proteins containing one or more of the following elements: His (MGSSHHHHHHSSLVPRGSH); His6 (MHHHHHH or HHHHHH for N- and C-terminal sequences, respectively); NLS (PKKKRKV); NLS' (PKKKKKV); T7 (MASMTGGQQMG); MTS (AAVLLPVLLAP); TAT (YGRKKRRQRRR) and (KFF)3K (KFFKFFKFFK). The table on the right lists the name, size (number of amino acids), yield following purification from E. coli (mg/L), relative solubility, and in vitro specific activity (units per milligram of enzyme) of each fusion protein. (B). Analysis of purified Cre fusion proteins. Purified Cre fusion proteins were fractionated by SDS-PAGE and stained with Coomassie blue.