Detecting imbalanced expression of SNP alleles by minisequencing on microarrays

BMC Biotechnology

Table 5 Test of allelic imbalance in replicate RNA-preparations and cDNA samples.

	cDNA/gDNA ratios in replicate RNA preparations ^a
	rs4331 ACE	rs1042719 ADRB2	rs5351 EDNRB
HUVEC	2.8 (1.4 – 4.0)	0.48 (0.34 – 0.62)	1.2 (1.1 – 1.4)
	rs5925 LDLR	rs5930 LDLR	rs1433099 LDLR
HAEC	1.3 (0.98 – 1.5)	1.6 (1.0 – 2.3)	1.5 (1.0 – 2.0)
	cDNA/gDNA ratios in replicate cDNA samples ^b
	rs5925 LDLR	rs5930 LDLR	rs1433099 LDLR
HAEC	1.5 (1.3 – 1.7)	1.9 (1.7 – 2.4)	1.1 (0.87 – 1.4)

Average cDNA/gDNA ratios from five replicate experiments with range of variation in parantheses.
^a cDNA synthesised from RNA extracted from five different cell batches analysed in duplicate using solid-phase minisequencing assays in a microtiter plate format.
^b Five replicate cDNA samples were reverse transcribed from the same RNA preparation and analysed in duplicate assays.

ISSN: 1472-6750