Table 3 Fluorescence signals obtained by genotyping six SNPs in RNA (cDNA) and genomic DNA from the HUVEC and HAEC cells using the two minisequencing methods.
From: Detecting imbalanced expression of SNP alleles by minisequencing on microarrays
Fluorescence signal (CV %)b | Fluorescence signal ratioc | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
cDNA | gDNA | cDNA | gDNA | ||||||||
SNPa | Method | Allele 1 | Allele 2 | Allele 1 | Allele 2 | ||||||
rs4331 | I | 17975 | (31%) | 4428 | (20%) | 17568 | (12%) | 10291 | (10%) | 4.1 | 1.7 |
ACE | II | 20447 | (37%) | 21796 | (11%) | 8928 | (35%) | 26913 | (18%) | 0.94 | 0.33 |
rs1042719 | I | 14368 | (12%) | 28538 | (25%) | 21179 | (11%) | 22192 | (12%) | 0.50 | 0.95 |
ADRB2 | II | 11475 | (3.6%) | 34346 | (5.4%) | 18495 | (7.4%) | 39569 | (8.6%) | 0.33 | 0.47 |
rs5351 | I | 11026 | (10%) | 1743 | (15%) | 7344 | (16%) | 2077 | (17%) | 6.3 | 3.5 |
EDNRB | II | 65257 | (0%) | 7484 | (14%) | 64360 | (3.3%) | 11552 | (20%) | 8.7 | 5.6 |
rs5925 | I | 13984 | (25%) | 16040 | (5.7%) | 9586 | (32%) | 9329 | (20%) | 0.87 | 1.0 |
LDLR | II | 3504 | (27%) | 9582 | (14%) | 1951 | (10%) | 7113 | (8.2%) | 0.37 | 0.27 |
rs5930 | I | 5680 | (13%) | 5028 | (13%) | 7207 | (22%) | 9244 | (17%) | 1.1 | 0.78 |
LDLR | II | 5410 | (31%) | 9965 | (15%) | 3261 | (27%) | 11183 | (9.0%) | 0.54 | 0.29 |
rs1433099 | I | 8806 | (20%) | 4594 | (10%) | 7307 | (32%) | 3646 | (23%) | 2.0 | 2.0 |
LDLR | II | 2550 | (41%) | 4507 | (16%) | 1727 | (36%) | 3743 | (13%) | 0.57 | 0.46 |