Table 1 Comparison of four imaging methods to readout the wound healing assay
Device | Resolution | Observable phenotypes | Timepoint for assaying | Acquisition speed | Cost of device |
|---|---|---|---|---|---|
Automated microscope | 3 μm/pixel | Decreased wound healing, aberrant morphology, increased mitotic index | 7 and 24 hr | ~1 hr/plate with 4× ~1.5 hr/plate with 10× | ~$200,000 |
Fluorescence scanner | 42 μm/pixel | Decreased wound healing | 7 and 24 hr | 26 minutes/plate | ~$50,000 |
Conventional scanner | 10 μm/pixel | Decreased wound healing | 7 and 24 hr | 8.5 minutes/plate | ~$1,000 |
Fluorescence macroscope | ~100 μm/pixel | Decreased wound healing | 24 hr | 5 seconds/plate | ~$500,000 |