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Table 1 Comparison of four imaging methods to readout the wound healing assay

From: A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods

Device Resolution Observable phenotypes Timepoint for assaying Acquisition speed Cost of device
Automated microscope 3 μm/pixel Decreased wound healing, aberrant morphology, increased mitotic index 7 and 24 hr ~1 hr/plate with 4×
~1.5 hr/plate with 10×
~$200,000
Fluorescence scanner 42 μm/pixel Decreased wound healing 7 and 24 hr 26 minutes/plate ~$50,000
Conventional scanner 10 μm/pixel Decreased wound healing 7 and 24 hr 8.5 minutes/plate ~$1,000
Fluorescence macroscope ~100 μm/pixel Decreased wound healing 24 hr 5 seconds/plate ~$500,000