The use of scanners to assay wound healing after 7 hrs recovery. (A) An image of actin-stained wells obtained using a fluorescence scanner. Normal healing wounds (left two columns) can be distinguished from wells treated with a titration of the actin cytoskeleton disrupter cytochalasin D (white box) by the width of the wound and staining at the wound margin. (B) An image of Coomassie-stained wells obtained using a transmitted-light scanner. Normal healing wounds have a more diffuse wound margin, denoting cell migration into the wound, than wells treated with cytochalasin D (white box). Bar 1 mm.