Template | Mutation | Mutagenic primer sequence | Megaprimer (bp) | Enzyme |
---|---|---|---|---|
E. coli KARI | E213D | 5'-GTCCTTCGTTGCGGAcAAATCTGACCTG-3' (F) | 968 | Nil |
A. thaliana AHAS Regulatory subunit | N346H | 5'-CGAAGGGGgTACcaTATCCAGAGC-3' (F) | 705 | KpnI |
S. cerevisiae AHAS Regulatory subunit | D224A | 5'-GGTCAAGTTGGTGATAgCGTT-3' (R) | 805 | EcoRV |
S. cerevisiae AHAS Catalytic subunit | M354V | 5'-CAACCGTGCAcACCAAGCATATCC-3' (R) | 1127 | ApaL1 |