Figure 3From: A new approach to 'megaprimer' polymerase chain reaction mutagenesis without an intermediate gel purification stepAgarose gel representing the results of the importance of prolonged extension cycle. Lane M contains 1 μg of GeneRuler™ DNA ladder mix and lane C shows the results of an experiment using 0.05 pmole of the first flanking primer but conducted without prolonged extension cycles.Back to article page