Multi-line split DNA synthesis: a novel combinatorial method to make high quality peptide libraries

BMC Biotechnology

Table 3 The comparison between the target amino acids composition and the actual composition of various libraries.

A. Uniform Type
Method	3NPs	mRNA display	MLSDS
Reference, Library	[29], 96T λ 6	[18], Random	This work, No Cys
Target value	Equimolar 19 amino acids	Equimolar 20 amino acids	Equimolar 19 amino acids
Correlation coefficient ^a	0.43	0.56	0.66
Sum of absolute errors ^b [%]	54.5	23.7	28.1
Percentage of stop codon	ND ^c	0.46	0.00
Percentage of cassettes containing stop codon	ND ^c	8.33	0.00
B. Doping Type
Method	3NPs	mRNA display	MLSDS
Reference, Library	[29], 100z	[18], β-Cassette polar	This work, c-Fos, e', g'-
Target value	Biased	Polar amino acid	Biased
Correlation coefficient ^a	0.94	0.56	1.00
Sum of absolute errors ^b [%]	42.4	73.7	5.66
Percentage of stop codon	ND ^c	0.39	0.00
Percentage of cassettes containing stop codon	ND ^c	6.25	0.00

The values were calculated using the data of full-length (without deletion) libraries. Values of 3NPs and mRNA display method were calculated with some assumptions. In mRNA display method, the value of stop codons were estimated from data described in the article and some assumptions; one cassette did not have two or more stop codons and stop codons appeared equally in every position. The target composition of β-Cassette polar is assumed that polar amino acids are equi-molar and the others are 0%.
^aCorrelation coefficient is calculated using target and actual amio acids composition including stop codons contribution. And it is assumed that stop codons were not appeared in full-length libraries of 3NPs method.
^b Sum of absolute values of difference between each target and actual amino acid.
^c ND is abbreviation of no data.

ISSN: 1472-6750