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Table 3 The comparison between the target amino acids composition and the actual composition of various libraries.

From: Multi-line split DNA synthesis: a novel combinatorial method to make high quality peptide libraries

A. Uniform Type    
Method 3NPs mRNA display MLSDS
Reference, Library [29], 96T λ 6 [18], Random This work, No Cys
Target value Equimolar 19 amino acids Equimolar 20 amino acids Equimolar 19 amino acids
Correlation coefficient a 0.43 0.56 0.66
Sum of absolute errors b [%] 54.5 23.7 28.1
Percentage of stop codon ND c 0.46 0.00
Percentage of cassettes containing stop codon ND c 8.33 0.00
B. Doping Type    
Method 3NPs mRNA display MLSDS
Reference, Library [29], 100z [18], β-Cassette polar This work, c-Fos, e', g'-
Target value Biased Polar amino acid Biased
Correlation coefficient a 0.94 0.56 1.00
Sum of absolute errors b [%] 42.4 73.7 5.66
Percentage of stop codon ND c 0.39 0.00
Percentage of cassettes containing stop codon ND c 6.25 0.00
  1. The values were calculated using the data of full-length (without deletion) libraries. Values of 3NPs and mRNA display method were calculated with some assumptions. In mRNA display method, the value of stop codons were estimated from data described in the article and some assumptions; one cassette did not have two or more stop codons and stop codons appeared equally in every position. The target composition of β-Cassette polar is assumed that polar amino acids are equi-molar and the others are 0%.
  2. aCorrelation coefficient is calculated using target and actual amio acids composition including stop codons contribution. And it is assumed that stop codons were not appeared in full-length libraries of 3NPs method.
  3. b Sum of absolute values of difference between each target and actual amino acid.
  4. c ND is abbreviation of no data.