Table 2 Comparison of the amino acid compositions of the actually synthesized DNA libraries (the full length library) with the target composition
From: Multi-line split DNA synthesis: a novel combinatorial method to make high quality peptide libraries
Library name | Natural | No Cys | c-Fos e', g'- | |||
|---|---|---|---|---|---|---|
| Â | Target | Actual | Target | Actual | Target | Actual |
Ala | 8.9% | 11.4% | 5.3% | 6.7% | 0.0% | 0.0% |
Arg | 4.7 | 3.5 | 5.3 | 5.9 | 30.0 | 29.7 |
Asn | 4.4 | 3.5 | 5.3 | 4.3 | 0.0 | 0.0 |
Asp | 5.5 | 8.6 | 5.3 | 5.9 | 0.0 | 0.0 |
Cys | 2.8 | 2.5 | 0.0 | 0.0 | 0.0 | 0.0 |
Gln | 3.9 | 2.1 | 5.3 | 5.5 | 20.0 | 19.6 |
Glu | 6.2 | 6.9 | 5.3 | 5.5 | 20.0 | 25.0 |
Gly | 7.8 | 12.0 | 5.3 | 8.2 | 0.0 | 0.0 |
His | 2.0 | 1.2 | 5.3 | 2.7 | 0.0 | 0.0 |
Ile | 4.6 | 3.9 | 5.3 | 5.9 | 0.0 | 0.0 |
Leu | 7.5 | 3.7 | 5.3 | 4.3 | 0.0 | 0.0 |
Lys | 7.0 | 6.2 | 5.3 | 4.7 | 30.0 | 27.9 |
Met | 1.7 | 1.1 | 5.3 | 3.5 | 0.0 | 0.0 |
Phe | 3.5 | 4.0 | 5.3 | 5.1 | 0.0 | 0.0 |
Pro | 4.6 | 2.5 | 5.3 | 2.4 | 0.0 | 0.0 |
Ser | 7.1 | 8.1 | 5.3 | 1.6 | 0.0 | 0.0 |
Thr | 6.0 | 5.8 | 5.3 | 6.7 | 0.0 | 0.0 |
Trp | 1.1 | 1.8 | 5.3 | 8.2 | 0.0 | 0.0 |
Tyr | 3.5 | 4.4 | 5.3 | 4.7 | 0.0 | 0.0 |
Val | 6.9 | 6.9 | 5.3 | 8.2 | 0.0 | 0.0 |
Term | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
Number of sequenced codon | - | 568 | - | 255 | - | 219 |
Correlation coefficient | - | 0.85 | - | 0.66 | - | 1.00 |
Sum of absolute errors | - | 26.9% | - | 28.1% | - | 5.66% |