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Table 2 Comparison of the amino acid compositions of the actually synthesized DNA libraries (the full length library) with the target composition

From: Multi-line split DNA synthesis: a novel combinatorial method to make high quality peptide libraries

Library name Natural No Cys c-Fos e', g'-
  Target Actual Target Actual Target Actual
Ala 8.9% 11.4% 5.3% 6.7% 0.0% 0.0%
Arg 4.7 3.5 5.3 5.9 30.0 29.7
Asn 4.4 3.5 5.3 4.3 0.0 0.0
Asp 5.5 8.6 5.3 5.9 0.0 0.0
Cys 2.8 2.5 0.0 0.0 0.0 0.0
Gln 3.9 2.1 5.3 5.5 20.0 19.6
Glu 6.2 6.9 5.3 5.5 20.0 25.0
Gly 7.8 12.0 5.3 8.2 0.0 0.0
His 2.0 1.2 5.3 2.7 0.0 0.0
Ile 4.6 3.9 5.3 5.9 0.0 0.0
Leu 7.5 3.7 5.3 4.3 0.0 0.0
Lys 7.0 6.2 5.3 4.7 30.0 27.9
Met 1.7 1.1 5.3 3.5 0.0 0.0
Phe 3.5 4.0 5.3 5.1 0.0 0.0
Pro 4.6 2.5 5.3 2.4 0.0 0.0
Ser 7.1 8.1 5.3 1.6 0.0 0.0
Thr 6.0 5.8 5.3 6.7 0.0 0.0
Trp 1.1 1.8 5.3 8.2 0.0 0.0
Tyr 3.5 4.4 5.3 4.7 0.0 0.0
Val 6.9 6.9 5.3 8.2 0.0 0.0
Term 0.0 0.0 0.0 0.0 0.0 0.0
Number of sequenced codon - 568 - 255 - 219
Correlation coefficient - 0.85 - 0.66 - 1.00
Sum of absolute errors - 26.9% - 28.1% - 5.66%
  1. "Term" denotes stop codons. In each target composition, [Term] = 0. "Natural": the library with the natural abundance (the average composition in 207 natural proteins) [18]; "No Cys": the library with uniform composition except [Cys] = 0; "c-Fos e', g'-": a c-Fos mutant library containing only 4 kinds of amino acid as same as in Ref.[26].