Table 1 Examples of molar mixing ratio of bases for MLSDS libraries
From: Multi-line split DNA synthesis: a novel combinatorial method to make high quality peptide libraries
Library name | No Cys | Natural | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Line Number of Synthesizers | Line-1 | Line-2 | Line-3 | Line-1 | Line-2 | Line-3 | ||||||||||||
ID Number of premix phosphoramidites | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
Molar mixing ratio of A:T:G:C | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â |
A | 2 | 3 | 5 | 5 | 0 | 0 | 2 | 5 | 0 | 5 | 4 | 2 | 2 | 0 | 0 | 3 | 4 | 0 |
T | 0 | 0 | 3 | 4 | 3 | 0 | 3 | 5 | 2 | 0 | 1 | 1 | 3 | 2 | 3 | 4 | 2 | 5 |
G | 3 | 0 | 0 | 4 | 3 | 1 | 1 | 0 | 0 | 5 | 0 | 2 | 5 | 5 | 2 | 4 | 0 | 0 |
C | 3 | 1 | 0 | 0 | 1 | 0 | 1 | 3 | 5 | 3 | 1 | 0 | 2 | 2 | 1 | 2 | 4 | 0 |