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Table 1 Effects of plant virus gene silencing suppressors on RNAi in Drosophila cells.

From: Heterologous expression of plant virus genes that suppress post-transcriptional gene silencing results in suppression of RNA interference in Drosophilacells

Cell Type

Transfection

% cells stained *

DS2

pMT/V5-His/lacZ

82.86 ± 9.12

DS2

pMT/V5-His/lacZ + dsRNA

17.70 ± 4.25

DS2

pMT/V5-His/lacZ + dsRNA + pMT-HC-Pro

45.78 ± 4.89

DS2

pMT/V5-His/lacZ + dsRNA + pMT-HC-Pro-K

14.62 ± 2.81

DS2

pMT/V5-His/lacZ + dsRNA + pMT-2b

11.13 ± 1.58

DS2

pMT/V5-His/lacZ + dsRNA + pMT-16K

47.25 ± 3.4

DS2-HC-Pro

pMT/V5-His/lacZ

42.04 ± 3.07

DS2-HC-Pro

pMT/V5-His/lacZ + dsRNA

31.70 ± 2.46

DS2-scAb S20

pMT/V5-His/lacZ

40.67 ± 2.36

DS2-scAb S20

pMT/V5-His/lacZ + dsRNA

6.50 ± 1.2

DS2-2b

pMT/V5-His/lacZ

41.16 ± 2.36

DS2-2b

pMT/V5-His/lacZ + dsRNA

9.17 ± 2.56

  1. * Four randomly selected fields of view each containing ~100 cells were selected in each of duplicate plates and the number of cells staining blue was counted for each experiment. The figures shown are the means from four experiments for the DS2 cells and three experiments for the DS2-HC-Pro and DS2-ScAb S20 cells.