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Table 1 Effects of plant virus gene silencing suppressors on RNAi in Drosophila cells.

From: Heterologous expression of plant virus genes that suppress post-transcriptional gene silencing results in suppression of RNA interference in Drosophilacells

Cell Type Transfection % cells stained *
DS2 pMT/V5-His/lacZ 82.86 ± 9.12
DS2 pMT/V5-His/lacZ + dsRNA 17.70 ± 4.25
DS2 pMT/V5-His/lacZ + dsRNA + pMT-HC-Pro 45.78 ± 4.89
DS2 pMT/V5-His/lacZ + dsRNA + pMT-HC-Pro-K 14.62 ± 2.81
DS2 pMT/V5-His/lacZ + dsRNA + pMT-2b 11.13 ± 1.58
DS2 pMT/V5-His/lacZ + dsRNA + pMT-16K 47.25 ± 3.4
DS2-HC-Pro pMT/V5-His/lacZ 42.04 ± 3.07
DS2-HC-Pro pMT/V5-His/lacZ + dsRNA 31.70 ± 2.46
DS2-scAb S20 pMT/V5-His/lacZ 40.67 ± 2.36
DS2-scAb S20 pMT/V5-His/lacZ + dsRNA 6.50 ± 1.2
DS2-2b pMT/V5-His/lacZ 41.16 ± 2.36
DS2-2b pMT/V5-His/lacZ + dsRNA 9.17 ± 2.56
  1. * Four randomly selected fields of view each containing ~100 cells were selected in each of duplicate plates and the number of cells staining blue was counted for each experiment. The figures shown are the means from four experiments for the DS2 cells and three experiments for the DS2-HC-Pro and DS2-ScAb S20 cells.