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Figure 3 | BMC Biotechnology

Figure 3

From: Heterologous expression of plant virus genes that suppress post-transcriptional gene silencing results in suppression of RNA interference in Drosophilacells

Figure 3

Northern blot analysis of lacZ RNA in DS2 cells. Upper Panels. Northern blot analysis of total cellular RNA probed with a lacZ-specific probe corresponding to ~500 bp of the 5' end of the lacZ gene. RNA was extracted from DS2 cells (lanes 1, 2, 7 and 9), DS2-HC-Pro cells (lanes 3 and 4), DS2-16k cells (lanes 5 and 6) or DS2-2b cells (lanes 8 and 10). All cells were transfected with pMT/V5-His/lacZ and lanes 2, 4, 6, 9 and 10 were co-transfected with pMT/V5-His/lacZ and dsRNA. The lacZ specific band migrated between 2661nt and 4742 nt digoxigenin labelled RNA size markers. Lower Panels – Stained gels showing loadings of Drosophila rRNA before blotting.

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