Expression of TRV 16k protein in Drosophila cells. Immunoblot of lysates from control cells (lanes 1,2 and 5,6), cells transiently transfected with pMT-16k (lanes 3,4) or cells stably transformed with pMT-16k (lanes 7,8). Cells represented in lanes 2, 4, 6 and 8 were induced by addition of CuSO4 and cells represented in lanes 1, 3, 5 and 7 were uninduced. Lanes 9 and 10 were lysates from control plant or plant infected with TRV respectively. Lysates were analysed by polyacrylamide gel electrophoresis, proteins transferred to nitrocellulose by electroblotting and probed with an anti-16k antibody. The position of migration of SeeBlue Plus 2 Pre-stained molecular weight markers (Invitrogen) bands is shown.