Skip to main content
Figure 3 | BMC Biotechnology

Figure 3

From: A faster way to make GFP-based biosensors: Two new transposons for creating multicolored libraries of fluorescent fusion proteins

Figure 3

Transposition of the type 1 IP 3 R with the Double Barrel Transposon (<DBT>). Transient expression in HEK-293 cells allows identification of in-frame insertions producing truncated GFP- (A) or DsRed- (B) IP3R fusion proteins. Digestion and subsequent re-ligation produces a full-length fluorescent IP3R fusion protein (C) (Scale bar = 20 μm).

Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us