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Figure 1 | BMC Biotechnology

Figure 1

From: Use and comparison of different internal ribosomal entry sites (IRES) in tricistronic retroviral vectors

Figure 1

Structure of retroviral constructs. TFG vectors are MFG-based retroviral plasmids into which the hCD70 cDNA and hCD80 cDNAs have been cloned, with either a neoR or a zeoR selectable marker gene. The three genes are co-translated from a tricistronic mRNA which was transcribed from the 5' long terminal repeat. The translation of the first cDNA (CD70 or CD80) depends on the 5' long terminal repeat-gag IRES, while the translation of the selectable marker and the second cDNA (CD70 or CD80) are dependent on two IRESes from different origins.

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