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Table 4 ΔCt values for progeny of heterozygous T1 line A443-1 (21 plants) in ascending order

From: Two-fold differences are the detection limit for determining transgene copy numbers in plants by real-time PCR

plant

ΔCt (Ct FAM-Ct TET) ± SDa

Containing the transgene according to Southern analysis (homo- and hemizygous)

A443-1-20

1.12 ± 0.35

A443-1-12

1.25 ± 0.20

A443-1-6

1.36 ± 0.33

A443-1-11

1.50 ± 0.08

A443-1-2

1.54 ± 0.17

A443-1-23

1.55 ± 0.06

A443-1-3

1.56 ± 0.23

A443-1-13

1.60 ± 0.20

A443-1-5

1.73 ± 0.06

A443-1-24

1.82 ± 0.61

A443-1-1

1.96 ± 0.25

A443-1-21

2.03 ± 0.08

A443-1-18

2.04 ± 0.22

A443-1-4

2.06 ± 0.22

A443-1-19

2.13 ± 0.10

A443-1-7

2.15 ± 0.23

no transgene according to Southern analysis

A443-1-9

5.08 ± 0.22

A443-1-8

5.25 ± 0.32

A443-1-14

5.98 ± 0.39

A443-1-15

6.36 ± 0.27

A443-1-22

7.25 ± 0.02

Controls

A340-5-1b

8.18 ± 0.04

wildtype 12

11.18 ± 2.09

  1. a average of three measurements b transformed line without T-DNA (T-DNA segregated out)