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Table 2 Summary of Table 1: Unambiguous copy numbers, for a line all four copy numbers must be the same: real-time PCR copy numbersa for plant 1 and 2, Southern copy numbers for plant 1 and 2.

From: Two-fold differences are the detection limit for determining transgene copy numbers in plants by real-time PCR

Calibrator A434-4-2

Calibrator A300-1-2

15% (2 o13 lines)

46% (6 of 13 lines)

  1. a a calculated copy number of less than 0.5 is considered to represent no transgene
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BMC Biotechnology

ISSN: 1472-6750

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