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Figure 1 | BMC Biotechnology

Figure 1

From: Quantitative promoter analysis in Physcomitrella patens: a set of plant vectors activating gene expression within three orders of magnitude

Figure 1

Schematic structures of the basal vectors created. The presented fragments were inserted into a modified pUC18 vector, where every construct contained the CaMV 35S terminator (35ST). (A) Firefly luciferase (FL) plasmids including the respective promoter of interest (P). The construct pluc contains no translation enhancer, the construct pluc-enh includes a modified start codon context (enh) and pluc-Ω implies the TMV omega element (Ω). (B) The Renilla luciferase (RL) plasmid Rluc was used as an internal control for transfection efficiency. The Renilla luciferase reporter gene was driven by a long version of the CaMV 35S promoter (35SP).

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