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Table 1 Comparison of the different injection and electroporation techniques. Intramuscularly injected mice are represented as IM and intra-arterially injected mice are represented as IA. Mice that were electroporated are identified with a + sign and mice that were not electroporated are identified with a - sign. The four different electrode types are identified with letters that correspond to the letters in figure 3. a mean % β-Gal expression = average value of [β-Gal activity (mg)/total protein (mg)]. b,c,d,e,f,g statistical significance (p < .05) between groups with differing superscripts. n = 7 for each treatment group.

From: Optimization of ectopic gene expression in skeletal muscle through DNA transfer by electroporation

Group #

Limb

Injection

EP

Electrode Type

Mean % β-Gal expressiona (+/- SD)

Mean % Area of Muscle Damage (+/- SD)

1

Left

IA CMVLacZ in 0.9% Saline

No EP

No EP

0.0475b ± 0.0377

 

2

Right

IA CMVLacZ in 0.9% Saline

+

A

0.4900h ± 0.0200

 

3

Left

IM CMVLacZ in 0.9% Saline

No EP

No EP

0.0200b ± 0.0082

 

4

Right

IM CMVLacZ in 0.9% Saline

+

A

7.790c,e ± 4.5254

5.6 ± 4.9

5

Right

IM CMVLacZ in 0.9% Saline

+

B

5.04c,d,f ± 2.4683

8.5 ± 4.9

6

Right

IM CMVLacZ in 0.9% Saline

+

C

3.6167d ± 2.2234

21.3 ± 6.1

7

Right

IM CMVLacZ in 0.9% Saline

+

D

11.650e,g ± 3.0645

3.4 ± 4.9

8

Right

IM CMVLacZ in 1.25% DMSO

+

A

8.190e,f,g ± 1.9986

 

9

Left

IM CMVLacZ in LipoFECTAMINE

No EP

No EP

0.0214b ± 0.0107

 

10

Right

IM CMVLacZ in LipoFECTAMINE

+

A

0.0414b ± 0.1912