Figure 2

Ectopic DNA expression decreases with increasing tissue damage following electroporation. LacZ expressing cDNA was electroporated into the gastrocnemius/soleus skeletal muscle. DNA expression was quantitated by measuring the amount of b-gal staining per area total muscle, and is reported as a percent of the gastrocnemius/soleus muscles. Tissue damage was measured and is reported as a percent of damage per total gastrocnemius/soleus muscle area. A second order polynomial was fitted to the data and it was found that there was a correlation coefficient of 0.826, which corresponds to a statistical significance of p < 0.01. This polynomial demonstrates that with increasing skeletal muscle damage, the amount of ectopic DNA expression decreases.