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Figure 3 | BMC Biotechnology

Figure 3

From: Activation of the control reporter plasmids pRL-TK and pRL-SV40 by multiple GATA transcription factors can lead to aberrant normalization of transfection efficiency

Figure 3

GATA-stimulated pGL3-CYP11A reporter gene activities normalized by 2 different Renilla control plasmids. COS-7 cells were transfected with pGL3-CYP11A, and combinations of GATA-expressing and Renilla luciferase-encoding plasmids. Both firefly and Renilla luciferase activities were measured using the Dual Luciferase Reporter assay system. pGL3-CYP11A firefly luciferase activity is normalized by Renilla luciferase activity and expressed as fold change (mean ± sem) compared with control cells transfected with pcDNA3 and the corresponding Renilla plasmid.

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BMC Biotechnology

ISSN: 1472-6750

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