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Figure 1 | BMC Biotechnology

Figure 1

From: Activation of the control reporter plasmids pRL-TK and pRL-SV40 by multiple GATA transcription factors can lead to aberrant normalization of transfection efficiency

Figure 1

Promoter regions of pRL-TK and pRL-SV40. The Renilla luciferase gene (Rluc) is driven by the herpes simplex virus thymidine kinase (HSV-TK) promoter or SV40 enhancer/early promoter. Consensus GATA binding sequences, (A/T)GATA(A/G), are present in the intron upstream of T7 promoter and within HSV-TK but not SV40 promoter. Deletion mutants of the two control plasmids (pRL-TK-d238 and pRL-SV40-d238) were constructed by removal of 238 base pairs between the HindIII and ScaI restriction sites in the intron.

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BMC Biotechnology

ISSN: 1472-6750

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