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Figure 2 | BMC Biotechnology

Figure 2

From: The Flp double cross system a simple efficient procedure for cloning DNA fragments

Figure 2

a) Strategy for cloning the lacZ fragment into pBA128. The PCR-amplified lacZ fragment containing terminal inverted FRT sites is incubated with plasmid pBA128 and Flp. After transformation of XL1-Blue competent cells, the DNA from blue colonies is analyzed by EcoRI enzyme digestion. Colonies containing the parent pBA128 plasmid are white in color (left) whereas the p128lacZ transformants are blue (right).

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