From: A fluorescent cassette-based strategy for engineering multiple domain fusion proteins
Cassette | Function |
---|---|
General | |
pCfvtx | Fundamental vector for rapidly inserting PCR products using a fluorescence assay to create basic cassettes |
pVentx | Venus for acquisition of fluorescence |
Protein purification | |
pHistx | 6xHis affinity tag |
pGsttx | GST affinity tag |
pHisventx | 6xHis affinity tag that also allows protein expression estimation by fluorescence |
pHisvengsttx | A double affinity tag using 6xHis and GST for improved purity and protein expression estimation by fluorescence |
Subcellular organelle targeting | |
pTattx | HIV TAT protein tranduction domain for targeting to the nucleolus or peptide-mediated delivery of proteins to the nucleus |
pIl4tx | Interleukin-4 signal peptide for secretion of target protein (alone) or for localization to the ER (with KDEL retention) |
pKdeltx | KDEL retention signal for localization the ER (with signal peptide) |
Organelle markers | |
pTatvtx | A fluorescent marker for the nucleolus |
pIl4venkdeltx | A fluorescent marker for the ER |