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Table 1 List of cassettes

From: A fluorescent cassette-based strategy for engineering multiple domain fusion proteins

Cassette

Function

General

pCfvtx

Fundamental vector for rapidly inserting PCR products using a fluorescence assay to create basic cassettes

pVentx

Venus for acquisition of fluorescence

Protein purification

pHistx

6xHis affinity tag

pGsttx

GST affinity tag

pHisventx

6xHis affinity tag that also allows protein expression estimation by fluorescence

pHisvengsttx

A double affinity tag using 6xHis and GST for improved purity and protein expression estimation by fluorescence

Subcellular organelle targeting

pTattx

HIV TAT protein tranduction domain for targeting to the nucleolus or peptide-mediated delivery of proteins to the nucleus

pIl4tx

Interleukin-4 signal peptide for secretion of target protein (alone) or for localization to the ER (with KDEL retention)

pKdeltx

KDEL retention signal for localization the ER (with signal peptide)

Organelle markers

pTatvtx

A fluorescent marker for the nucleolus

pIl4venkdeltx

A fluorescent marker for the ER