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Table 2 PPV detection in non-inoculated apical leaves one week and six weeks after inoculation. DAS-ELISA absorbance values from ihprolC-PP197 transgenic and wild type plants are reported.

From: Expression of self-complementary hairpin RNA under the control of the rolCpromoter confers systemic disease resistance to plum pox virus without preventing local infection

  Lines
  8   13   29   2   9  
Plant 1 week 6 weeks 1 week 6 weeks 1 week 6 weeks 1 week 6 weeks 1 week 6 weeks
1 0.160 0.360* 0.061 0.002 0.012 0.002 0.056 0.001 0.097 0.023
2 0.129 0.080 0020 0.012 0.012 0.298* 0.044 0.010 0.089 0.027
3 0.119 0.069 0.054 0.041 0.005 0.086 0.112 0.038 0.079 0.031
4 0.126 0.066 0.020 0.022 0.070 0.008 0.092 0.075 0.047 0.018
5 0.094 0.355* 0.060 0.083 0.003 0.002 0.137 0.004 0.052 0.014
6 0.081 0.067 0.062 0.009 0.011 0.005    0.117 0.001
7 0.057 0.072    0.006 0.424*    0.131 0.086
8 0.049 0.036    0.008 0.873*    0.104 0.047
9 0.080 0.032        0.387 0.021
10 0.084 0.082         
11 0.075 0.640*         
WT 0.720   1.520   0.650   0.450   0.450  
  1. Samples with absorbance values higher than 0.097 were considered virus-infected. This threshold was calculated as mean absorbance value of the mock inoculated plants plus 3 times the standard deviation of the mean.* Transgenic plants showing leaf mottling (i.e. mild symptoms of PPV infection).