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Figure 3 | BMC Biotechnology

Figure 3

From: Expression of self-complementary hairpin RNA under the control of the rolCpromoter confers systemic disease resistance to plum pox virus without preventing local infection

Figure 3

Northern blot analysis of ihprolC-PP197 and rolC-PP2x197 transgenic plants. Panel A. RNA blot of the ihprolC-PP197 mRNA in N. benthamiana transgenic lines 2, 29, 8, 13 and 9 (lanes 2, 3, 4, 5 and 6, respectively) and control untransformed plant (lane 1). Panel B. RNA blot of rolC-PP2x197 mRNA in N. benthamiana transgenic lines 4, 6 and 19 (lanes 2, 3 and 4, respectively) and control untransformed plant (lane 1). The blots were hybridised to a DNA probe corresponding to a 484 bp long sequence at the 3' end of the ihprolC-PP197 gene. The ihprolC-PP197 and rolC-PP2x197 transcripts were detected after 3 days and 12 h of autoradiography, respectively. The membranes were also hybridised to an actin probe and exposed for 12 h. Both probes were labeled with (α-32P)-dCTP (3000 Ci/mmol) using "Ready to go DNA labeling beads (-dCTP)" (Amersham Biosciences); 106 cpm/ml of labeled probe were added to the hybridization buffer.

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