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Table 1 Spectroskopic properties and pKa of Sapphire mutants.

From: Efficiently folding and circularly permuted variants of the Sapphire mutant of GFP

  λ (ex) λ (em) QY ε pKa
Sapphire 399 511 0.64 25000 4.9
T-Sapphire 399 511 0.6 44000 4.9
cpT-Sapphire 145-144 399 511 0.14 32000 5.6
cpT-Sapphire 174-173 399 511 0.53 44000 5.2
  1. L(ex) is the peak of the excitation spectrum in units of nm. L (em) is the peak of the emission in units of nm. QY is the fluorescence quantum yield, which is dimensionless. E is the extinction coefficient in units of M-1cm-1.