Table 1 Spectroskopic properties and pKa of Sapphire mutants.
From: Efficiently folding and circularly permuted variants of the Sapphire mutant of GFP
λ (ex) | λ (em) | QY | ε | pKa | |
|---|---|---|---|---|---|
Sapphire | 399 | 511 | 0.64 | 25000 | 4.9 |
T-Sapphire | 399 | 511 | 0.6 | 44000 | 4.9 |
cpT-Sapphire 145-144 | 399 | 511 | 0.14 | 32000 | 5.6 |
cpT-Sapphire 174-173 | 399 | 511 | 0.53 | 44000 | 5.2 |