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Figure 4 | BMC Biotechnology

Figure 4

From: In situtransduction of target cells on solid surfaces by immobilized viral vectors

Figure 4

Effect of the natural permissivity of target cells for adenoviral infection on in situ transduction of the cells by adenoviral vectors immobilized on a solid surface. A, D-17 cells (canine osteosarcoma cell line that is highly permissive to adenoviral infection); B, C6 cells (rat glioma cell line that is poorly permissive to adenoviral infection). Ad5.CMV-LacZ was treated with 15 μg/ml sulfo-NHS-LC-biotin, followed by the removal of non-virion-associated biotinylation reagent. Varying numbers of the resulting biotinylated adenoviral vectors were added to streptavidin-coated wells (well diameter, 0.64 cm; Reacti-Bind Streptavidin Coated Polystyrene Wells) for immobilization, followed by the removal of unbound viral particles. Target cells (8 × 103 cells per well) were placed on the wells and cultured at 37°C for 48 h. Cells were fixed with glutaraldehyde and stained for the expression of the lacZ gene (). Unmodified Ad5.CMV-LacZ was used free in solution as a control (). Data shown in A and B are representative of three and four, respectively, independent experiments.

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