Figure 4
From: In situtransduction of target cells on solid surfaces by immobilized viral vectors
Effect of the natural permissivity of target cells for adenoviral infection on in situ transduction of the cells by adenoviral vectors immobilized on a solid surface. A, D-17 cells (canine osteosarcoma cell line that is highly permissive to adenoviral infection); B, C6 cells (rat glioma cell line that is poorly permissive to adenoviral infection). Ad5.CMV-LacZ was treated with 15 μg/ml sulfo-NHS-LC-biotin, followed by the removal of non-virion-associated biotinylation reagent. Varying numbers of the resulting biotinylated adenoviral vectors were added to streptavidin-coated wells (well diameter, 0.64 cm; Reacti-Bind Streptavidin Coated Polystyrene Wells) for immobilization, followed by the removal of unbound viral particles. Target cells (8 × 103 cells per well) were placed on the wells and cultured at 37°C for 48 h. Cells were fixed with glutaraldehyde and stained for the expression of the lacZ gene (●). Unmodified Ad5.CMV-LacZ was used free in solution as a control (○). Data shown in A and B are representative of three and four, respectively, independent experiments.