In situ transduction of target cells by adenoviral vectors immobilized on a solid surface. Ad5.CMV-LacZ was treated with varying concentrations of sulfo-NHS-LC-biotin, followed by the removal of non-virion-associated biotinylated reagent. The resulting viral vectors (5 × 106 viral particles per well) were incubated in streptavidin-coated wells (well diameter, 0.64 cm; Reacti-Bind Streptavidin Coated Polystyrene Wells) for 2 h at 25°C for immobilization. After unbound viral particles were removed, D-17 cells (8 × 103 cells per well) were placed in the wells and cultured at 37°C for 48 h. Cells were fixed with glutaraldehyde and stained for the expression of the lacZ gene (●). Biotinylated Ad5.CMV-LacZ (5 × 106 viral particles per well) was used free in solution as a control (○). Data shown are representative of six independent experiments.